Measure GABA

 

Method of GABA Measurement

 

For this GABA assay samples are deproteinated and amino acids are derivatized prior to analysis by liquid chromatography tandem mass spectrometry (LC/MS/MS). A standard curve is produced with a concentration range of 5-5000 ng/ml GABA. Each sample is spiked with an internal standard for quantification.   A water blank negative control and known concentrations of GABA positive controls are included in each assay.

 

Sample Preparation

 

  • ♦ Use EDTA or heparin as an anticoagulant to collect plasma (usually green top vacutainer) or red top serum separator. Within 30 minutes of drawing sample centrifuge for 15 minutes at 1000   x g at 2 – 8°C. Store samples at  -80°C prior to shipping. Avoid freeze-thaw.
  • ♦ If you send conditioned media for testing, remember the limit of detection is ~5 ng/ml.
  • ♦ The assay requires 150-200 microliters of sample. This allows for repeat measures if necessary. Contact us if the amount of sample is less, we can work with you.
  • ♦ Label samples with your identification number and the time and date that it was drawn. If you are measuring area under the curve, the exact time samples were drawn (not 90 mins for example, but 8:35 AM) should be on label and in file.
  • ♦ Let us know on the form, if possible, when plasma samples were drawn relative to feeding.

 

Examples of standard curves.

GABA Both Standard curves

 

Packing Samples

  • ♦ Pack on plenty of dry ice and seal the cooler lid with packing tape so specimens remain frozen should the shipment be delayed.
  • ♦ Ship overnight no later than Wednesday, the lab is closed on weekends.
  • ♦ Pack specimens in sample boxes, plastic bags often break in contact with dry ice.
  • ♦ Include and email quantify@mitokine.com a copy of the sample inventory and order form. This will help us confirm that all shipments are complete.
  • ♦ Please email quantify@mitokine.com or phone (207) 422-6838 when you ship so that someone is available to receive samples.

 

Human Samples

If you are sending human samples please send the IRB approval to quantify@mitokine.com.

Frequent questions:  http://mitokine.com/faq/

Print order form:  ‎http://mitokine.com/fax-form/

Shipping and Contact

 

Mitokine Bioscience, LLC

Analytical Laboratory

Suite 1 Tamarack Place

13 Captain Bill Road

Hancock, ME 04640

 

(207) 422-6838    fax: (207) 422-6830

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Contact Us
Interested in learning more? Feel free to contact us.